Distribution of KPC-2 producing strains at different sampling sites and with selective pressure applied.

Abstract

Please refer to Fig 1 for plant configuration and stage abbreviations. The numbers of isolates was represented as the CFUs per 100 μL samples from each sampling site. Species identification of these isolates was determined by 16S rRNA gene sequencing (% similarity): Klebsiella spp. (99.3%-99.6% identity to K. oxytoca strain JCM1665); Enterococcus spp. (97.4%-98.5% identity to E. faecium strain gp34); Acinetobacter spp. (98.2%-99.3% identity to A. seohaensis strain SW-100); Escherichia spp. (99.7%-99.9% identity to E. coli O157); Shigella spp. (99.4%-99.8% identity to S. sonnei strain CECT4887); Stenotrophomonas spp. (98.2%-98.8% identity to S. maltophilia R551-3); Wautersiella spp. (97.5%-98.1% identity to Wautersiella sp. MBG55); and Paenibacillus spp. (99.0%-99.2% identity to Paenibacillus sp. 1–9).</p