Histological findings of BMMNC retention 5 min after IC injection.

Abstract

Recipient hearts were collected at 5 minutes after IC injection of 8x106 BMMNC that had been labeled with PKH67. A and B represent low- and high-magnification images of Isolectin-B4 staining, respectively (scale bar = 100 μm [A] and 30 μm [B]). Representative micro images from n = 4 hearts are presented. All donor BMMNC (green) retained in the heart were found to be entrapped in isolation and within the coronary microvasculature. Blue signals for nuclei (DAPI); red for endothelial cells (Isolectin-B4).</p