Retinal vasculature development into the deep and intermediate layers of wild type and <i>Fzd4</i>^+/- mice under normoxic conditions.

Abstract

Whole mount retinas of Fzd4+/+ and Fzd4+/- under normoxic conditions were stained with isolectin-594 and imaged at 25x magnification using a Zeiss LSM 510 Meta laser scanning confocal microscope. Z-stacks representing vascular growth in the deep and intermediate layers at the mid periphery were generated using 1 micron slices. Z-stacks were analyzed using the ImageJ software program. Green bars represent the X-axis, blue bars represent the Y-axis and purple represent the Z-axis. White arrows indicate examples of vessel branching in retinal layers.</p