LANA binds to the entire KSHV genome and is required for the recruitment of EZH2 onto the viral episome during <i>de novo</i> infection.

Abstract

(A) Immunoblot analysis of LANA expression in BAC16-3xF-LANA infected SLK cells at 72 hpi using anti-LANA and anti-FLAG antibodies. (B and C) FLAG and LANA ChIP assays for testing LANA-binding to viral promoters in BAC16 and BAC16-3xF-LANA KSHV-infected SLK cells at 72 hpi. Cellular MYT1 promoter was used as a control. (D) ChIP-on-chip analysis of the genome-wide binding of LANA and EZH2 to the KSHV genome in WT and LANA KO KSHV-infected cells at 72 hpi.</p