EZH2 and RelB are Required for the Maintenance of TNBC TIC.

Abstract

(A) Top panel, Quantification of tumorspheres formed by 100 SUM-149 or MDA-MB-231 cells expressing control siRNA or siRNA targeting EZH2 or RelB. Values given as means ± standard deviation from three biological repeats. *P<0.05, **P< 0.005 versus controls; two tailed unpaired t-test. Bottom panel, Immunoblots of indicated proteins in adherent SUM-149 or MDA-MB-231 cells expressing the indicated siRNA construct. β-actin serves as a loading control. (B) Quantification of tumorspheres formed by either 100 SUM-149 or MDA-MB-231 cells treated daily for 3 days with 1μM UNC1999. Values given as means ± standard deviation from three biological repeats. **P< 0.005 versus controls; two tailed unpaired t-test.</p