Expression of Engrailed-1 and M-cadherin during early avian development.

Abstract

Whole mount in-situ hybridisation showing developmental expression of Engrailed-1 (En-1) (A1-A4) and M-cadherin (M-cad) (B1-B4) at HH14, HH22, HH24 and HH27. At HH-14 En-1 expression was only confined to the midbrain-hindbrain junction (indicated by asterisk (*) in A1). At HH22 (A2 and B2) and HH24 (A3 and B3), En-1 expression is limited along the dorsal-ventral axis compared to M-cad (red arrow). Furthermore, En-1 is not expressed in developing skeletal musculature of the head (black arrow), tongue muscle (blue arrow) and limb muscle (green arrow) (A3-A4). In contrast to En-1, M-cad is expressed in skeletal musculature of the head (black arrow), tongue muscle (blue arrow) and limb muscle (green arrow) (A4-B4).</p