Buccal swabs as a non-destructive tissue sampling method for DNA analysis in amphibians

Abstract

ISI Document Delivery No.: 821GR Times Cited: 6 Cited Reference Count: 14 English Article 0268-0130International audienceThis study describes a non-destructive DNA sampling method for genetic studies on amphibians using buccal swabs. We assessed the quantity and quality of DNA collected in each species by amplifying a part of the cytochrome b gene (381-1060 bp) and microsatellite markers. Buccal swab sampling is a useful alternative method for DNA sampling for both mtDNA and nDNA markers in amphibians. However, only frozen storage allowed microsatellite genotyping. We conclude that this method could greatly increase the accessibility of genetic studies in small vertebrates and could be preferred in the field of conservation genetics