Wide hybridization in Arachis: problems and prospects

Abstract

The genus Arschis includes a large number of apdu including A. hypoped, the groundnut. The species are grouped into men wctions. A. hypog@ad. a tetraploid, can be crowed only with the sped08 within its own ssction, Amchis, even though most of them arr diploids. From the diploid species resistances are being trms?md to A. hypogma. Investigations on barriers to hybridization betwoen sections have been initiated at ICRISAT. Soms techniques have bean dmloped to break the barriers. After incompatible poll~nations betw*en A. h-1) or A. monticols and species in section Rhiromatosw. fluoremncrr microscopic investigation reveals that some inhibition of pollen tubs growth occurs in the style. However, pollen tubes can be soen at the base of the pistils. In very few instances pegs do develop but do not elongate further than 1 cm and degenerate before they can penetrate the mil and form pods. Applications of growth hormones to the bases of incompatibly pollinated flowers just afm pollination significantly in~asedth e number of peg6 produced from about 10 percent (untreated) to mom than 60 percent (hormone treated) of pollinations. Hormones were clp~li~sdin gly or in combination. or in sequencr. GA3 induced moat pep. but thou died bdorr aoda weto formed. A wbowuent treatment with kinetin maintained peg growth to a stage at which embryo$ could k disuc?ed from immrturo pods and succe#fuliy cubrod in virt

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