DEL AGUILA, E. M.

PASCHOALIN, V. M. F.

PINTO JUNIOR, W. R.

ROSENTHAL, A.

SILVA, J. T.

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Industrial and Manufacturing (Track)USE OF A METALLOPROTEASE GENE (APRX) AS A MARKER TO IDENTIFY PSEUDOMONAS SP. CONTAMINATION IN A DAIRYPLANTW. R. Pinto Junior, E. M. Del Aguila, J. T. Silva, A. Rosenthal and V. M. F. PaschoalinBioquímica, Universidade Federal do Rio de Janeiro, Brazil.Abstract:To prevent bacterial deterioration of dairy products, a rapid test for detection of Pseudomonas sp. strains high in proteolytic activity in milkis useful. Protease digestion of milk can lead to clotting and gelation of milk casein. A conventional plate-counting procedure to detectpsychrotrophic contamination in milk products is time-consuming and not useful to prevent food degradation. The aprX gene encoding analkaline metalloprotease is considered the responsible agent for milk spoilage. PCR methods targeting for this sequence can accelerate thedetection process. To identify Pseudomonas sp. isolates able to express and produce the aprX enzyme, 15 strains collected from a dairyplant were analyzed after culturing in specific Pseudomonas medium. DNA sequencing of 16S rDNA and aprX regions was performed, andtwo genus Pseudomonas sp. (P. chlororaphis, P. panacis, P. japonica, P. fluorescens) and Stenotrophomonas maltophilia were identified andisolated from four surfaces in the cheese-processing plant (refrigerated milk storage tank before and after cleaning, cheese-processingequipment,  and cheese-making mold).  This  study revealed several  Pseudomonas species able to colonize a dairy plant and with thepotential to degrade and spoil food substances with a high protein content.Keywords: aprx gene, pseudomonas, milk.Copyright @ 2012 Global Biotechnology Congress 2013

Use of a metalloprotease gene (APRX) as a marker to identify pseudomonas sp. contamination in a dairy plant.

http://www.alice.cnptia.embrapa.br/alice/bitstream/doc/981309/1/2013210.pdf

Use of a metalloprotease gene (APRX) as a marker to identify pseudomonas sp. contamination in a dairy plant.

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