红锥(CastanopsishystrixA.DC.),属壳斗科常绿乔木,具有生长快、材质优、适应广等优良特性。本实验目的是通过ISSR分子标记、过氧化物和酯酶同工酶、rbcL基因序列差异比对，从不同角度探讨10个优质红锥品系的遗传多样性，并且结合重要生理指标进行综合分析。主要实验结果如下：(1)通过ISSR对10个优质红锥品系亲缘关系分析：实验确定红锥ISSR-PCR最佳扩增条件为20ul反应体系中:模板60ng,1UTaq酶,1.5mmol/LMg2+,ｄNTP0.25mmol/L,引物0.5μmol/L，2％甲酰胺。PCR扩增条件:94℃预变性5min,94℃变性30S,52~55℃复性...Castanopsis hystrix A.DC. is a kind of evergreen arbors having many eximious characters such as：growing fast,excellent texture,abroad acclimatization.In this study，inter-simple sequence repeat(ISSR)、peroxidese isozymes、esterase isoenzymes、analysis of rbcL gene sequences were evaluated for its potential use in the genetic variability of 10 cultivars of Castanopsis hystrix A.DC.，and then combined w...学位：理学硕士院系专业：生命科学学院生物学系_细胞生物学学号：20032607
