以锯缘青蟹（Scyllaserrata）内脏为材料，通过含0.2mol/LNaCl的0.01mol/LTris-HCl（pH7.5）缓冲液抽提、硫酸铵分级沉淀、SephadexG-100凝胶柱过滤层析、DE-32离子交换柱层析，获得了比活力为7,990U/mg的电泳单一纯N-乙酰-β-D-氨基葡萄糖苷酶制剂（NAGase,EC3.2.1.52）。该酶的等电点为4.68，分子量约为132kD，每个酶蛋白分子含有约1097个氨基酸残基，其中非极性占39.1%,碱性占7.1%，酸性占24.0%，极性不带电荷的占29.8%。该酶含两个分子量约为65.8kD的同型亚基，肽链间不含二硫键而肽链内含有二硫键...N-Acetyl-β--D-glucosaminidase (NAGase, EC 3.2.1.52) was purified from viscera of green crab (Scylla serrata), by extraction with 0.01 mol/L Tris-HCl buffer (pH 7.5) containing 0.2 mol/L NaCl and ammonium sulfate fractionation, then chromatography on Sephadex G-100 and DEAE-cellulose (DE-32). The purified enzyme was a single band on polyacrylamide gel electrophoresis with specific activity to be 7,...学位：理学博士院系专业：生命科学学院生物化学与生物技术系_生物化学与分子生物学学号：B20032602
