本文研究EDF的分离纯化方法及其对MEL细胞的诱导分化。 以哺乳类动物（兔）为材料，按费氏法分离纯化EDF，即给新西兰兔 注射盐酸苯肼致贫血，收集网织红细胞，用冻融法裂解细胞，乙醇、 氯仿变性除去血红蛋白，过SephadexG-100，超滤浓缩后再过 DEAE——纤维素和CM——纤维素柱，获得纯化30，000倍的活性物质EDF， SDS－PAGE电泳为单一蛋白带，分子量15KD，产率9．6％。另将 过sephadexG-100后的活性浓缩法直接过CM-柱，也得到活性组 分，其结果与费氏法相似。或将上述浓缩液直接等电聚焦层析；也得 到相近的结果。 观察EDF（纯品）对MEL细胞...This research includes two parts: 1) the isolation and purification of EDF and its method improvement; 2) the differentiation of MEL cells treated by EDF. EDF were isolated and purified with Fei’s method. First, New Zealand rabbits were made anemic by subcutaneous injection of phenylhydrozine hydrochloride. Blood collected from anemic rabbits was washed by saline to remove leukocytes, and reticul...学位：理学硕士院系专业：生命科学学院生物学系_细胞生物学学号：xy00012
