花生四烯酸（Arachidonicacid，ARA）是人体一种必需的多不饱和脂肪酸，它 不仅在营养代谢中起重要作用，而且还被广泛应用于医药、食品和饲料等各种领 域，有着广阔的应用前景。但是传统的发酵工艺并不能满足日益增加的市场需求， 需要从分子水平进行改造以提高ARA的发酵生产水平。 本文首先以高山被孢霉的总RNA为模板，通过PCR反转录技术扩增出高山被 孢霉延长酶基因（GLELO），将其克隆到simpleTVector，构建重组质粒TGLELO 。由于GLELO序列上有含有BamHI酶切位点，与要插入pD4质粒所用酶切 位点一致，需敲除GLELO基因上的BamHI酶切位点，实验...Arachidonic acid (ARA) is one of the essential polyunsaturated fatty acids in human, which plays an important role in nutrition metabolism. ARA has a wide application prospect in the field of medicine, food industry and feed additive. While ARA yield produced by traditional fermentation strategy cannot meet the increasing market demand, it is necessary to improve the production of ARA through ...学位：工学硕士院系专业：化学化工学院化学工程与生物工程系_生物化工学号：2062009115129
