Poster Session: Virology and Viral ReceptorsBackground: Swine has been considered an animal reservoir of pandemic influenza A virus (IAV), for
example, the 2009 H1N1 pandemic virus, swine is acting as a “mixing vessel” for the reassortment of
swine, human and avian IAVs. Certain influenza B virus (IBV) strains were also found to be readily
infecting piglets as early as in 1969. However, tissue tropism of IBV in swine is understudied, at least
in 2000s, mainly due to the misconception that IBV causes milder disease than IAV. IBV has in fact
circulated in many parts of the world causing regular seasonal epidemics in humans with mortality
rates sometimes higher than that in IAV seasons. Here, our research group hypothesizes that swine
could be a neglected host of IBV, apart from human and seal, due to the previous infectivity of IBV in
this animal, as well as the fact that swine has close contact with human and possesses a similar sialic
acid (influenza virus receptor) distribution profile as the human respiratory tract. We aim to examine
the characteristics of IBV tissue tropism using swine tracheal and lung explant models, and risk
assess swine susceptibility to a panel of IBV strains from both Yamagata and Victoria lineages of
different years. Materials and Methods: The tracheal and lung explants were prepared from fresh
swine respiratory organs from approximately 6-month-old pigs, and cultured with maximal similarity to
the in vivo conditions. A panel of IBV strains, from both Yamagata and Victoria lineages and from
different years, were used to infect the tissue explants at 37oC or 39oC according to the original
physiological temperature of the tissue. The virus replication efficiencies were evaluated through
viral titration and immunohistochemistry of the collected supernatant and formalin-fixed tissue
explants respectively at 1, 24, 48 and 72 h postinfection. Seasonal IAVs (H1N1 - A/OK/447/08
and H3N2 - A/OK/370/05) were used as controls. Results: Most of the tested IBVs showed
productive replication in the swine lung explants. Swine tracheal explants, on the other hand,
supported the replication of limited IBV strains. Most of these IBVs belong to the Victoria lineage,
which spread across the years from 2005 to 2011. IBVs that could replicate in swine lung explants
reached their maxima at 48 hpi or sometimes later. This is comparatively slower than the
replication rates of seasonal IAVs (H1N1 & H3N2) used in the study, which usually showed
significant increase at 24 hpi with still increasing virus yields at 48 hpi in some cases. However, the
overall increase in titres between the IBVs and seasonal IAVs were similar. In swine tracheal
explants, both IBVs and seasonal IAVs showed limited replications with similar trends of having
maxima being reached at 24 hpi. Conclusions: The successful replication of IBVs in swine explants
cultures indicates the possible susceptibility of swine to IBV and provides the essential basis for
further investigation on the likelihood for swine to be an animal reservoir of the virus, as well as the
threat it may pose to humans. Continuous studies on the replication kinetics of a greater number of
IBVs in swine explant cultures across a wider range of years, countries and lineages will probably be
our future target.published_or_final_versio
