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Routine and sensitive SPE-HPLC method for quantitative determination of phenophytin a and pyropheophytin a in olive oils

Abstract

3rd International Congress on Pigments in Food: pigments in food, more than colours (Quimper, Brittany, France, 14-17th of june, 2004)In the present study we have developed a combine SPE and HPLC, that can be considered as a modification of a previous one (5) in two ways: first assuring a total recovery of pheophytin a and pyropheophytin a during SPE sample extraction, and secondly, improving selectivity of detection and sensitivity of quantification by using fluorescence detection.This work was financially supported by the Dirección General de Investigación of the Ministry of Science and Technology (MCYT, Spanish Government), project AGL2000-0699, and the Secretaría General de Universidades e Investigación (Consejería de Educación y Ciencia, Junta de Andalucía).Peer reviewe

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