Isolation and Characterization of microsatellite loci in Tripterygion delaisi.

Abstract

2 páginas, 1 tabla.We isolated 49 microsatellite loci from a genomic library of Tripterygion delaisi × anthosoma enriched for CA and GA repeats. Ten loci were screened in 30 individuals with high numbers of alleles per locus (averaging 15.5 ± 2.86) and observed heterozygosity (averaging 0.765 ± 0.052). No deviations from Hardy–Weinberg expectations were detected. These highly polymorphic markers will be useful in determining the spatial patterns of genetic diversity between and within subspecies of Tripterygion delaisi .This research was supported by a Predoctoral fellowship from the Ministerio de Educación, Cultura y Deporte to J.C. (AP2001-0225). Research was funded by projects REN2001-2312 and BOS2000-0295 of the MCYT and EVK3-CT-2000-00041, supported by the EC.Peer reviewe