The fate of lignin in water and sediment of the Garonne river (France) and of a pond in its floodplain was examined using specifically labeled [14C-lignin] lignocelluloses. No significant differences appeared in the mineralization rate of alder, poplar or willow [14C-lignin] in running water samples. Conversion of total radi- oactivity to 14C02 ranged between 18.7 and 24.4% after 120 days of incubation. Degree of 14C-Iabeled lig- nin mineralization in standing water and sediments was clearly lower, especially in submerged sediments, and was correlated with oxygen supply. After 60 days of incubation 3.3% to 7.9% of the 14C-Iabeled lignin was recovered in water samples as dissolved organic carbon originating from microbial metabolism. In water ex- tracts from sediment the percentage of dissolved organic 14C was only 0.4% to 1.3% of the applied activity. In the humic fraction extracted from sediments it did not exceed 4.4% which was much lower than in soils. No significant difference appeared between river and pond conditions for humic substances formation