P-37 Bioinstrumentation of Electrochemiluminescent Sensor Using Cell Phones

Abstract

An escalating need for emergency or point-of-care diagnostics drive the biosensor to be simple to make, portable, affordable while still providing reliability and sensitivity of a high-end equipment. A multipurpose electrochemiluminescence (ECL) based biosensor is being developed utilizing ubiquitous mobile technologies. Mobile technology’s ubiquity and connectivity plays a pivotal role in simplifying biosensor instrumentation and bringing it closer to end users. ECL biosensor is a great platform to satisfy the need for ultrasensitive immunoassay development and ultra- compact instrumentation. ECL reagent of Ru(bpy)32+ complex was used and its inherently low signal was overcome by co-reactant such as DBAE. Screen printed electrode and paper based fluid channel enabled pump-less flow cell. Full waver DC circuit to rectify the AC voltage from audiojack of mobile phone and amplify it up to 6V is developed. The ECL reaction was triggered by the cell phone powered voltage and the ECL signal was detected by the cell phone camera. The signal generated by mM levels of Ru(bpy)32+ complex and DBAE were strong enough to be detected by naked eyes. The signal intensity correlated with the reactant concentration linearly. The project is currently seeking external funding