thesis

Molecular Genetic Characterization of Endemic Yellow Catfish, Horabagrus brachysoma (Gunther)

Abstract

Horabagrus brachysoma (Gunther) an endemic, cultivable catfish belonging to Family Bagridae is found in rivers originating from southern part of the biodiversity hotspot - the Western Ghats. This species is categorized as "endangered" based on latest IUCN criteria, due to restricted distribution, loss of habitat, over exploitation, destructive fishing practices and trade (Anon., 1998). ... The species is shonlisted for taking up stock-specific, propagation-assisted rehabilitation programme in rivers where it is naturally distributed. Information on the basic genetic profile and stock structure, which is essential for the fishery management, conservation and rehahilitation of this species, is lacking. Hence, the present work was undenaken to identify molecular genetic markers like allozymes, RAPD and microsatellites in H. braclrysoma and to use them in analyzing the population genetic structure of this species, collected from three geographically isolated river systems viz., Meenachil, Chalakkudy and Nethravathi in South India. ... In allozymes, the screening was carried out to detect 25 enzymes, but only 14 showed their presence with scorable activity. Out of founeen enzymes studied, 12 enzymes were polymorphic and two enzymes were monomorphic. The founeen enzymes yielded 25 scorable loci in all populations. A total of 33 alleles were obtained in overall populations

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