The secondary metabolites of seaweed Ulva fasciata and Hypnea musciformis,collected form southeast and southwest coast of India.

were tested for biotoxicity potential. Both species bowed potent

activity in antibacterial, brine shrimp cytotoxicity, larvicidal, antifouling

and ichthyotoxicity assays

Joseph, Selvin

Lipton, A P

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Journal of Marine Science and Technology, Vol. 12, No. 1, pp. 1-6 (2004) BIOPOTENTIALS OF ULVA FASCIATA AND HYPNEA MUSClFORMlS COLLECTED FROM THE PENINSULAR COAST OF INDIA Joseph Selvin* and Aaron Premnath Lipton** Key words: seaweeda, secondary metabolite. U11.tr ftrsc.ictrtr. Hypr~rir ~i~rr.si~~fi)r~rr~.s, antibacterial. brine shrimp cytotoxicity, larvicidal. antifouling. ichthyotoxic. ABSTRACT The secondary metabolite5 of seaweed\ Ul~~tr,fir.sc~itrtrr and Hyprrerr tr~rc\i~ifi)rnzis, collected form southeast and southwest coast of India. were tested for biotoxicity potential.  Both \pecies  \bowed potent activlty in antibacterial,  brine shrimp cytotoxicity. larvicidal,  anti-  fouling and ichthyotoxic i ty  a s w y s .  T h e  green a lga  U. f'cr.\c.itrrtr exhibited broad-spectrum antibacterial actlvity wherea\ the red alga H. rtlrr.\c.iforrili. showed narrow spectrum antibacterial activity. The brine \hrirnp cytotoxicity profile indicated that the seaweeds  were moderately toxic .  The  overall  activity profile indicated that U. fa.scinttr contained more biological potency than H. rinrrc~iforri~i.~. INTRODUCTION Marine algae are not only the primary and major producers of organic matter in the sea, but they also exert profound effects on the density and distribution of other inhabitants of the marine environment. An under- standing of the wide range of behavioural relationships that exist among organisms would provide us with clues to substances of biomedical interest. Marine secondary metaboli tes are organic  c o ~ n p o u n d s  produced by microbes,  sponges ,  seaweeds ,  and other  mar ine  organisms. The host organism biosynthesizes these compounds as non-primary or secondary metabolites to protect themselves and to maintain homeostasis in their environment. Some of these secondary metabolites offer avenues for developing cost-effective, safe and potent drugs. Nearly 5 0  lakhs (50,000,00) species available in the sea are virtually untapped sources of secondary metabolites. Those compounds already iso- lated from seaweeds are providing valuable ideas for the development of new drugs against cancer, microbial infections and inflammation (Elena et a / . ,  2001 ; Kim et trl.. 1997; Okai et nl.. 1997; Premila et a / . ,  1996) apart from their potential ecological/industrial significances such as controlling reproduction, settlement/biofouling and  feed ing  deter rents  (Se lv in ,  2002) .  In th is  background, the present study intended to evaluate the toxic influence, activity range and potency of seaweeds collected from the peninsular coast of India. MATERIALS AND METHODS The algae, which inhabited exclusively on the intertidal rocky substratum, were selected for the col- lection in order to avoid other algal contamination (Selvin, 2002). Predominant algae such as Ulva jusciata and H?pnea milscijorrnis were collected along the south- east and southwest of India. Immediately after collection, they were washed in fresh seawater to remove the epiphytes, sand and other extraneous matter and air- dried under shade. Completely dried material was weighed and ground finely in a mechanical grinder. The secondary metabolites of seaweeds were extracted in bulk extraction process. In this process, 500 g of finely powdered algal material was refluxed in a 5 L capacity round bottom flask using methanol-dichloromethane (1 : 1). The extract was filtered and concentrated to recover the excess solvents in another distillation system. Finally it was reduced to thick oily natured crude extract in a rotary vacuum evaporator (Buchi) at 40°C. Modified cylinder plate double layer method was used for the evaluation of antibacterial potential of seaweeds. The following bacterial strains were used as test organism (i) Seven type cultures (obtained from Microbial Type Culture Collections,  Chandigargh,  India): Micrococcus lccteus (ML), Bacillus cereus (BC), Bacillus subtilis (BS), Escherichia coli (EC), Aeromonus hydrophila (AH), Pseudomonas aeruginosa (PA),  and Vibrio jisclzeri (VF),  (ii) two shrimp isolates: Vibrio cr1ginoljticu.s (QS7) and Vibrio hurveyi (RJMS) (iii) two fish isolates: Aeromorzas sp. (CF- 1 )  and Aeromonas sp. 

Biopotentials of Ulva fasciata and Hypnea musciformis collected from the peninsular coast of India

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Biopotentials of Ulva fasciata and Hypnea musciformis collected from the peninsular coast of India

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