[原著］Prethymic Nylon Wool-Passed Bone Marrow Cells Can Make Distinction between Self and Non-Self X-Chromosome-Linked Gene Products (Xir Antigens) on the Stimulator Cells, Resulting in Regulation of the Generation of Cytotoxic T Lymphocytes in Mixed Lymphocyte Cultures

Abstract

We have previously reported that nylon wool-passed bone marrow cells treated with anti-Thy.1 antibody and complement (Thy.1 NW-BM cells) had helper-like activity which could augment the generation of cytotoxic T lymphocytes (CTL). In this study, we determined the antigens to which these NW-BM cells responded and recognized. When a few responder lymph node (LN) cells and an excess of NW-BM responder cells from BIOBR (H-2^k B10 background) mice were cultured with stimulator spleen cells from either B10D2 (H-2^k, B10 background) or BALB/c (H-2^d, BALB/c background) mice, the number of CTL induced by the stimulation with BALB/c spleen cells was significantly higher than in those from B10D2 mice. When the cells from BALB.K (H-2^k. BALB/c background) mice were used as the responder, the results showed that B10D2 spleen cells were better stimulators than those of BALB/c. These results showed that NW-BM cells responded to the nonself allogeneic background gene products on the stimulator cells, but not to those of self, and that these responder NW-BM cells augmented CTL generation. We genetically analyzed the antigens that NW-BM cells recognized among many products derived from background genes. B10BR NWBM cells were well stimulated by (BALB.KXB10D2) F1 male spleen cells, but not by those of (B10D2×BALB.K) F1 males. On the contrary, BALB.K NW-BM cells were highly stimulated by (B10D2XBALB.K) F1 males, but not by (BALB.KXB10D2) F1 males. These results showed that NW-BM c?lIs had a strict preference for X-chromosome linked gene products (Xir antigens). We also showed that the augmenting ability of NW-BM cells was attributed to the Thy.1 population, and that BALB/c NW-BM cells possessing the H-2^d haplotype also responded preferentialy to nonself Xir antigens, To conclude, NW-BM cells could distinguish between self and non-self Xir antigens, resulting in regulation of the CTL response.論文http://purl.org/coar/resource_type/c_650