The calcium hydroxide ionization of four root canal sealers (Sealapex, CRCS, Sealer 26, and Apexit) was studied by measuring conductivity and pH and by conducting atomic absorption spectrophotometry. Samples 6 mm in diameter and 15 mm long were prepared from these sealers. After setting and 48 h storage in a desiccator, five samples of each material were placed in 50 mL distilled water and analysed after 0,1,2,4, 6 and 24 h and 5, 15 and 30 days. The results showed that Sealapex was the root canal sealer showing the highest pH, ionic calcium and total calcium values (P<0.05) throughout the experimental period, followed by CRCS, Apexit and Sealer 26.Department of Pediatric Dentistry Ribeirão Preto Dental School USPDepartment of Endodontics Araraquara Dental School UNESPDepartment of Physical Chemistry Ribeirão Preto Sch. of Pharm. USPDepartment of Endodontics Araraquara Dental School UNESP, Rua Humaitá, 1680, CEP 14801-903, Araraquara, SPDepartment of Endodontics Araraquara Dental School UNESPDepartment of Endodontics Araraquara Dental School UNESP, Rua Humaitá, 1680, CEP 14801-903, Araraquara, S