We present the design and optimization of a benchtop NMR
spectrometer for real-time metabolic monitoring of 3D tissue on
microfluidic platforms, utilizing hyperpolarization via dynamic
nuclear polarisation. We show the modifications made to a
commercial benchtop NMR spectrometer, the design and
fabrication of a microfluidic platform ensuring consistent
injection of hyperpolarized substrates and ongoing cell media
renewal, and its integration with a radio frequency (RF) coil for
data transmission and reception (Tx/Rx). Additionally, the
construction of a sample carrier is presented. Preliminary NMR
results from this system are also provided.This work has received funding from: The European
Union’s Horizon 2020 research and innovation program
(GA-863037); the Spanish grants with reference PID2020-
117859RA-I00 funded by
MCIN/AEI/10.13039/501100011033 (NARMYD),
RYC2020-029099-I funded by
MCIN/AEI10.13039/501100011033 and by “ESF
Investing in your future”, PLEC2022-009256 funded by
MCIN/AEI/10.13039/501100011033 and by the
“European Union NextGenerationEU/PRTR”; The BIST
(Barcelona Institute of Science and Technology)-“la
Caixa” Banking Foundation Chemical Biology
programme
