NMR metabolite profiling analysis of pigmented rice resistance to rice ear bug

Abstract

Rice ear bugs or Leptocorisa oratorius F. are major rice pests reported to reduce the rice yields severely. The present study aimed to screen local pigmented cultivars for resistance to rice ear bugs and perform metabolomics analysis to identify the metabolites responsible for the resistance character. For screening, eight cultivars including both black and red varieties were used. Rice ear bugs used for bioassay were collected from the field. The assay results showed that two cultivars of black rice and one cultivar of red rice exhibited high resistance characters as per the Standard Evaluation System guidelines for rice. Metabolite profiling of rice seeds was performed at the milky stage using 500 Mhz NMR JEOL, followed by multivariate analysis with SIMCA ver 14. Metabolite profiling identified nine out of 15 metabolites, which were significantly different between the most resistant and susceptible cultivars. In the red rice, hydroxy-L-proline, threonine and formic acid and for black rice, valine, glutamate, α-glucose, β-glucose, galactinol and raffinose were identified as potential metabolites conferring the resistance character. This study identified the most resistant cultivars which can be used in the future to support the development of a novel line of cultivar resistant to rice ear bug. © 2021 World Research Association. All rights reserved

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