Vaccination and follow-up schedule for study groups (created by authors using the licensed program BioRender (http://www.biorender.com).

Abstract

Dogs without documented previous rabies vaccination (grey) received their primary vaccination (T0, grey). For group 1 follow-up blood samples were taken seven months (T7), around one year (T12-14) and 1.5 years (T18) after primary vaccination. Due to logistic restrains the follow-up sequence differs (blue) within this group between dogs from Kandal (n = 16) and dogs from Battambang (n = 205). From dogs of group 2, a blood sample (T12) was collected one year after their primary immunization to document the immune response of this primary immunization. Afterwards these dogs received a booster vaccination (green), and additional samples were collected (green) more than one year (T26) and three years (T34) after the booster vaccination to monitor its effect on the immune response.</p