Frequency of VENUS⁺ cells after transfection of EVA cell cultures.

Abstract

EVA cells were co-transfected with the designated WT or mutant pCMV-nsP2 plasmids or an identical control plasmid lacking the methionine start codon for nsP2 (vector) and pCMV-VENKL. At 24 h post-transfection, cells were evaluated by flow cytometry for expression of VENUS. (A) Representative flow cytometry plots for vector (black, circle), nsP2 (red, circle) and nsP2QMS (blue, circle) and frequency of VENUS+ cells among live, CD45- cells. (B) Representative flow cytometry plots for vector (black, circle), nsP2 (red, circle), nsP2K192A (red, triangle), nsP2C478S (red, square) and nsP2QMS (blue, circle) and frequency of VENUS+ cells among live, CD45- cells. (EPS)</p