E2 and VENUS are equally detectable in CHIKV-infected EVA cells at 24 hpi.

Abstract

EVA cells were inoculated with 106 PFU of CHIKV-VENKL for 24hpi, harvested, fixed, permeabilized, and stained with anti-E2 CHIKV (CHK-11) at decreasing (2-fold) serial dilutions. Cells were analyzed for E2 and VENUS expression by flow cytometry. (A) Representative flow cytometry plots. (B) Quantification of cell frequency for E2 and VENUS positive cells (red = infected, black = mock). (EPS)</p