Purification and assembly of protein complexes.

Abstract

(A) Assembly of S. coelicolor RNAP-σHrdB. The peak of RNAP-σHrdB was analyzed by SDS-PAGE. (B) Purification of AfsR. (C) Purification of ΔTPR. (D) Purification of SARP. (E) Purification of RbpA. (F) Purification of CarD. (G) Purification of AfsKΔC. (H) Assembly of AfsRT337A-TIC in the presence of 1 mM ATPγS. The protein compositions in the dotted line boxed fractions are shown in the SDS-PAGE. The original gel images can be found in S1 Raw Images. (TIF)</p