Olaparib inhibits oxLDL-induced monocyte adhesion and foam cell formation.

Abstract

(A) THP-1 monocytes were exposed to oxLDL, the PARP inhibitor olaparib (Olapa), or the NLRP3 inhibitor MCC950 (MCC) as indicated. The monocytes attached to human umbilical vein endothelial cells (HUVECs) were counted using an inverted light microscope and analyzed by ImageJ software (NIH, Bethesda). Data are from four independent experiments (n = 4/group). *P<0.05 vs vehicle, #P<0.05 vs oxLDL only, one-way ANOVA, Newman-Keuls test. (B) THP-1 cells were exposed to PMA followed by compounds as in (A) as described in Methods section. Formation of foam cells was assayed by oil red-O staining and imaged by an inverted light microscope. ORO, oil red-O staining. Data are from three independent experiments (n = 3/group). Median and SD values are shown. *P<0.05 vs vehicle, #P<0.05 vs oxLDL only, one-way ANOVA, Newman-Keuls test.</p