(A-C) Legends for expected topology (A), protein length (B), and construct epitopes (C). (D) Protein topology schemes for the 15 constructs used. Below is a list with detailed information about the respective topology scheme of each construct depicted in (B). Uniprot accession numbers (acc.nr.) are available under https://www.uniprot.org/uniprot/. Sequences of all constructs are listed in “plasmid_sequence_information.xlsx” stored in “Plasmid_design.zip” available from http://dx.doi.org/10.17169/refubium-40101. No protein, used for background signals. (TIF)</p

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