Ferrini, F

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Institutional Research Information System University of Turin

26/12/2019 EventPilot Webhttps://ep70.eventpilot.us/web/page.php?page=IntHtml&project=FENS18&id=abstract_35349 1/1D045 - Chloride homeostasis modulates synaptic plasticity inthe superficial dorsal horn of the spinal cordView Session Detail to add to ScheduleD045 - Chloride homeostasis modulates synaptic plasticity in the superficial dorsal horn of thespinal cordJimena Perez Sanchez 11CERVO-Laval University, Cellular and Molecular Neurosciences, Quebec, CanadaFrancesco Ferrini 22University of Turin, Department of Veterinary Sciences, Turin, ItalyYves De Koninck 33CERVO-Laval University, Cellular and Molecular Neurosciences, Quebec, CanadaThe efficacy of GABAA/glycine-mediated inhibition in the adult brain relies on the capacity of centralneurons to extrude chloride (Cl-) to maintain a low intracellular Cl- concentration. In neurons, Cl-extrusion is critically dependent on the K+-Cl- cotransporter 2 (KCC2). However, the expression ofKCC2 is not homogeneous throughout the CNS. This heterogeneity may affect how different neuronsintegrate synaptic inputs and, thereby, how they express synaptic plasticity. We have previously foundthat the expression of KCC2 follows a distribution gradient across the superficial dorsal horn (SDH),with low levels of KCC2 in lamina I and higher levels in lamina II. To explore if differences in Cl-homeostasis across the SDH affect synaptic plasticity, we recorded field postsynaptic potentials(fPSPs) evoked by dorsal root stimulation, at different depths in the SDH from a whole spinal cordpreparation. After a stable baseline, long-term potentiation (LTP) was induced by low frequencystimulation (LFS: 2Hz, 2min) to the same dorsal root. To explore the role of KCC2 in the propensity ofLTP, we bath applied the selective KCC2 antagonist VU0240551 (10 μM). We found that LTPdeveloped faster and stronger in superficial recordings, where KCC2 levels are low, while LTP wasincreasingly weaker in deeper recordings. VU0240551 application abolished the gradient in LTPacross the SDH, suggesting a direct involvement of KCC2. These experiments suggest that chloridehomeostasis modulates the propensity for synaptic plasticity, as higher KCC2 activity is directly linkedwith reduced LTP in the SDH.This page uses cookies to improve your experience. Learn More OK Home Schedule Notes and Stars Search More ..

Chloride homeostasis modulates synaptic plasticity in the superficial dorsal horn of the spinal cord

https://iris.unito.it/bitstream/2318/1720407/1/EventPilot%20Web.pdf

Chloride homeostasis modulates synaptic plasticity in the superficial dorsal horn of the spinal cord

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