Antoniotti, Paola

Barge, Alessandro

Beccari, Fabio

Benzi, Paola

Boscaro, Valentina

Canepa, Carlo

Gallicchio, Margherita

Marabello, Domenica

Peira, Elena

English

Institutional Research Information System University of Turin

14 October 2023AperTO - Archivio Istituzionale Open Access dell'Università di TorinoOriginal Citation:EFFECT OF THERANOSTIC PHOSPHOLIPIDIC SRCL2/SRI2AND β-D FRUCTOPYRANOSE BASEDNANOPARTICLES ON CELL VIABILITY AND UPTAKETerms of use:Open Access(Article begins on next page)Anyone can freely access the full text of works made available as "Open Access". Works made availableunder a Creative Commons license can be used according to the terms and conditions of said license. Useof all other works requires consent of the right holder (author or publisher) if not exempted from copyrightprotection by the applicable law.Availability:This is the author's manuscriptThis version is available http://hdl.handle.net/2318/1891490 since 2023-02-09T11:38:59Z Introduction Nanoparticles can be engineered with precise sizes, shapes, composition and with surface targeting ligands that allow a specific interaction with target cells. The aim of this work was to analyze the effect of non-toxic and low-cost Sr/I-based nanoparticles, in order to develop a new 89Sr- and/or 131I-based radiotherapy method on cell lines. In particular we analyzed the effect on cellular uptake and cell viability of compound 1 (89Sr(fructose)2Cl2 · H2O) and compound 2 (89Sr(fructose)2I2 and Sr (fructose)2131I2), on a non-tumour cell line, hTERT-HME1, and a tumour cell line, HT-29. The compounds studied exhibit some features that could be interesting for biomedicine applications, such as the biocompatibility due to their non-toxic components and the significant SH emission, that can permit exploitation for in vitro bio-imaging.  Materials and methods We tested the effect of nanoparticles encapsulated in a hydrophilic shell of mPEG-DSPE that increases their biocompatibility on non-tumour and tumour cell lines, hTERT-HME1 and HT-29, respectively. In particular, we analyzed the cellular uptake of nanoparticles and the effect on cell viability. To evaluate the effect of nanoparticles on cell viability, we treated hTERT-HME1 and HT-29 cell lines with five different nanoparticles concentrations, and cell viability was assessed using ATP content and the CellTiter-Glo® Luminescent Cell Viability Assay (Promega). Results hTERT-HME1 cell line showed sensitivity to compound 1 already at the lowest concentration, while compound 2 reduced hTERT-HME1 cell viability in a dose-dependent manner; HT-29 viability was reduced of about 70% and 50% only at the highest concentration of nanoparticles of compounds 1 and 2, respectively. The nanoparticles covered with mPEG-DSPE can be detected for few days in a biological medium and can be internalized into hTERT-HME1 and HT-29 cells. Discussion and Conclusions  In this work we have analyzed the effect of Sr/I-based nanoparticles, on cell uptake and viability. The nanoparticles formulation could be useful to concentrate the effect of 89Sr and 131I radio-API in a specific tissue, allowing a selective activity of the activated compounds. The nanoparticles are biocompatible and are internalized into the cell; the viability of cell lines utilized is influenced by the presence of the nanoparticles themselves rather than their chemical composition.   ha formattato: Inglese americano

EFFECT OF THERANOSTIC PHOSPHOLIPIDIC SRCL2/SRI2AND β-D FRUCTOPYRANOSE BASED NANOPARTICLES ON CELL VIABILITY AND UPTAKE

https://iris.unito.it/bitstream/2318/1891490/1/SrI-SrF.pdf

EFFECT OF THERANOSTIC PHOSPHOLIPIDIC SRCL2/SRI2AND β-D FRUCTOPYRANOSE BASED NANOPARTICLES ON CELL VIABILITY AND UPTAKE

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