Analysis of <i>Apc</i> mRNA expression in the small intestinal tumors of B6/B6-Chr18^MSM-F1 Apc^<i>Min/+</i> mice.

Abstract

Quantitative PCR was performed using Gapdh as an endogenous control. Each point represents Apc mRNA level in an individual tumor (0 Gy Unidentified, n = 14; 2 Gy Unidentified, n = 15; 2 Gy Deletion, n = 6; normal intestinal tissue, n = 4). Primers were set to span exons 7 and 8 (A) and exons 16 and 17 (B) according to the NCBI database (NM_001402727.1). The means and standard deviations are represented by bars and error bars, respectively. Apc expression in normal intestinal tissue was set to 1. The triangle among the 2 Gy-Unidentified data points represents Apc expression in Tumor ID 2-2-23, in which a deletion comprised approximately 3.5 kbp at the end of the last exon of Apc. ** p (TIF)</p