<i>Apc</i> genomic region analyzed in this study.

Abstract

Nucleotide positions are numbered relative to the transcription start site of Apc (NM_007462). Capital letters represent CpG islands obtained from the UCSC Genome Browser (http://genome.ucsc.edu/). The CpG sites are highlighted in red. Primer pairs (boxed sequences) were designed using MethPrimer (http://www.urogene.org/cgi-bin/methprimer/methprimer.cgi). Arrows indicate the direction from 5’ to 3’. Due to the relative length of this CpG island, it was split into two segments, and bisulfite sequencing was performed for each segment. (TIF)</p