Bisulfite sequencing of the <i>Apc</i> promoter region in small intestinal tumors of B6/B6-Chr18^MSM-F1 <i>Apc</i>^<i>Min/+</i> mice.

Abstract

Methylation status of the 93 CpG dinucleotides in the promoter region of Apc. Each circle represents a CpG site in the genomic DNA sequence and each row of circles represents the analysis of a single cloned allele. Closed circles: methylated CpG dinucleotides; open circles: unmethylated CpG dinucleotides. Bisulfite efficiency averaged 98.5 ± 1.33% for CpG island 1 and 98.8 ± 1.23% for CpG island 2 in all samples examined.</p