Additional file 1 of Population mixing mediates the intestinal flora composition and facilitates invasiveness in a globally invasive fruit fly

Abstract

Additional file 1: Supplementary Information for results. Supplementary Information Fig. 1. Differences in invasiveness between the original (Fujian and Hainan) and mixed outbred populations (invasive population) of B. dorsalis. A, pupal weight; B, ovary size at 15 d after emergence; C, number of eggs laid per female per day; D, hatching rate. Supplementary Information Fig. 2. The effects of amino acid and intestinal microbe on body weight of the oriental fruit fly. A, Cephalo-pharyngeal bone length in inbred and outbred populations of B. dorsalis. B, Food intake of B. dorsalis inbred population and outbred population. C, pupal weight of inbred populations supplemented with different amino acids. D, pupa weight after feed exchange between inbred and outbred populations. Supplementary Information Fig. 3. Phenotypic differences between inbred and outbred populations of B. dorsalis. A, pupal weight; B, ovary size at 15 d after adult emergence; C, survival fraction; D, fecundity. Asterisks indicate significant differences (*,p<0.05; **, p<0.01, ***, p<0.001), and ns indicates no significant differences. Supplementary Information Fig. 4. Species compositions of the microbiomes of the intestinal flora and oviposition fluids in F populations of B. dorsalis. A, bacterial composition and relative abundance; B, fungal composition and relative abundance. The inner circle represents the oviposition fluids, and the outer circle represents the intestinal flora. Supplementary Information Fig. 5. Species compositions of the microbiomes of the intestinal flora in the inbred F and the outbred F♀×H♂ populations of B. dorsalis. A, bacterial composition and relative abundance; B, fungal composition and relative abundance. The inner circle represents the inbred F population, and the outer circle represents the outbred F♀×H♂ population. LEfSe diagram of intestinal bacteria (C) and fungi (D) between inbred F and outbred F♀×H♂ populations. Supplementary Information Fig. 6. Species compositions of the microbiomes of the intestinal flora in the inbred H and the outbred H♀×F♂ populations of B. dorsalis. A, bacterial composition and relative abundance; B, fungal composition and relative abundance. The inner circle represents the inbred H population, and the outer circle represents the outbred H♀×F♂ population. LEfSe diagram of intestinal bacteria (C) and fungi (D) between inbred H and outbred H♀×F♂ populations. Supplementary Information Table 1. The artificial diet of Bactrocera dorsalis. Supplementary Information Table 2. Life table parameters of the inbred and the outbred populations ofB. dorsalis. Supplementary Information for methods. Microbiome determination and analysis: DNA extraction and sequencing. Analysis of microbiome data. Diet exchange experiments