Bhalla, Nikhil

Ho, Chih‐Ching

Liu, Hui‐Ling

Tsai, Hsieh‐Fu

Yang, Chia‐Ming

English

The debye length is a measure of the distance over which the electric field of a charged particle decays in an electrolyte solution. If the binding of the analyte to the surface of the transducer is too far away from the surface, the electric field to the analyte may decay over a distance greater than the debye length thereby reducing the sensitivity of the measurement. In this context, this study has developed a simple one‐step protein immobilization strategy to covalently attach proteins on the sensor surface. Our binding strategy, which uses hydrogen peroxide (H2O2) ensures that the analyte is attached as close as possible to the transducer surface. This study evaluates our findings by comparing our strategy with silane chemistry and elucidating the debye length effects with colorimetric assays and field effect devices. Additionally, as a case study, we also evaluated the performance of our methodology for the detection of glucose oxidation by a field effect device. Overall, the developed immobilization strategy avoids the effects of the debye length and improves the performance of the biosensor

Ulster University's Research Portal

Single‐Step Primary Amine Synthesis on Proton Sensitive Nanofilms to Overcome Its Debye Length Limitations

https://pure.ulster.ac.uk/files/122334447/admi.202300080.pdf

Single‐Step Primary Amine Synthesis on Proton Sensitive Nanofilms to Overcome Its Debye Length Limitations

Abstract

Similar works

Full text

Available Versions

Ulster University's Research Portal