Taxol partially rescues MTs from XopL_Xe.

Abstract

Confocal microscopy of lower epidermal cells of GFP-TUA6 (labels MTs) stable transgenic N. benthamiana leaves. Leaves were agroinfected (OD600 of 0.4) to express (A-C) XopLXe-mCherry, (D-F) XopLm-mCherry and (K-M) mCherry and treated with the MT stabilizing chemical taxol (solved in DMSO) at 4 hpi. Samples were harvested for microscopy 2 dpi. The GFP channel is visible in white (labeled MTs) and the mCherry channel in magenta. Plastids are in cyan; ‘n’ labels nuclei. Scale bars are 20 μm. (G), (H), (I), (J) Are magnified images from (A), (B), (D) and (E), respectively (area magnified is outline with a white box). Scale bars in (G-J) are 5 μm. Examples of MTs are labeled with white arrows. (TIFF)</p