BACKGROUND: South Africa launched 24-hour movement guidelines for birth to 5 years in 2018. Perceptions of these guidelines were assessed as part of the dissemination process with community-based organizations in 2019. METHODS: Fifteen dissemination workshops were held with community-based organization representatives and a range of stakeholders. Discussions were held with workshop attendees (n = 281) to obtain qualitative feedback on the guidelines and workshop. Six follow-up focus groups (n = 28) were conducted to obtain additional feedback on the guidelines and their dissemination. Discussions and focus groups were thematically analyzed. RESULTS: Participants recognized the importance of the guidelines for the health and development of young South African children. Participants' perceptions of the guidelines were consistently positive. The participants acknowledged the alignment of the guidelines with other South African programs and initiatives, and that they addressed gaps. Screen time and sleep were identified as the behaviors needing particular attention among young South African children. The negative impact of COVID-19 on young children's movement behaviors was acknowledged, especially regarding screen time. CONCLUSION: These findings provide evidence of stakeholders' positive perceptions of the South African guidelines and support the dissemination and implementation of these guidelines for the promotion of early childhood health and development in South Africa.This study was funded by the Cambridge-Africa ALBORADA Research Fund, with supplementary funding from the International Society of Behavioral Nutrition and Physical Activity. The work of EMF van Sluijs was supported by the Medical Research Council [Unit Programme number MC_UU_00006/5], and undertaken under the auspices of the Centre for Diet and Activity Research (CEDAR), a UKCRC Public Health Research Centre of Excellence. Funding from the British Heart Foundation, Cancer Research UK, Economic and Social Research Council, Medical Research Council, the National Institute for Health Research, and the Wellcome Trust, under the auspices of the UK Clinical Research Collaboration, is gratefully acknowledged [087636/Z/08/Z; ES/G007462/1; MR/K023187/1]