The effects of CS and Oligo-CS on mRNA expression of marker genes.


C2C12 cells were grown in differentiation medium containing the indicated concentrations of CS or Oligo-CS for 72 hours. Then, the cells were harvested, and the total RNA isolated from the cells was subjected to real-time RT-PCR analysis using the specific primer set for each of the genes. The data indicate relative expressions compared to untreated control cells after normalization with the GAPDH mRNA expression. Error bars indicate standard deviations (n = 3). Asterisks (*: p p < 0.001) indicate that the difference is statistically significant compared to the control.</p

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