Zinc-65 was produced in the Nuclear Research Center for Agriculture and Medicine (NRCAM) by the bombardment of natural copper targets with 30 MeV protons via the 65Cu(p,n)65Zn nuclear reaction. Natural copper was used instead of enriched 65Cu because of the quick decay of undesired radioisotopes. It was also more desirable for cost effectiveness. Cross-section calculations were performed by ALICE nuclear code and the results were compared with the experimental data given in the literature, which showed good agreement. A 160 mi m copper layer target was bombarded with a 150 miA current of 30 MeV protons for 20 min, which resulted in 170 MBq activity of 65Zn product. The yield was 3.4 MBq/miAh. The concentration of the product was 6.8 MBq/ml. Radiochemical separation was carried out by anion exchange chromatography with the yield of about 98%. Quality control of the final product showed a radionuclide purity of more than 98% and no traces of possible impurities (copper) were detected by a colorimetric method with a 1 ppm detection limit using dithizone as the reagent. The materials used for targetry and chemical separation were quite cost-effective