The antibiotic sensitivity pattern and plasmid profile of Escherichia coli, Vibrio and Salmonella species isolated from well and river water sources in Oproama Community were investigated. Antibiotic sensitivity profiles of the bacteria (Escherichia coli, Vibrio sp. and Salmonella sp.) isolated from the water showed high sensitivity to oflaxicin, nalidixic acid and nitrofurantoin and high resistance to amoxicillin, augumentin, cotrimazole and tetracycline. Multi antibiotic resistant index (MARI) as high as 0.375 (Escherichia coli: E9; Vibrio spp.: V3, V4, V10; Salmonella spp.: S1, S9), 0.5 (Vibrio spp.: V2; Salmonella spp.: S2, S4) and 0.75 (Salmonella spp.: S7) were recorded after curing the plasmids with sodium deodecyl sulphate (SDS). The plasmid profiles revealed that 60% of the isolates harboured detectable plasmids with sizes up to 23.130 kb.Keywords: Antibiotics, Escherichia coli, Plasmid, Salmonella, Vibri