Drought is one of the abiotic constraint in rice production, which occured during plant growth. Wild rice with drought tolerance charactes may be expected to contribute drought tolerance in modern rice varieties. The study was conducted in green house of Indonesian Agricultural Biotechnology and Genetic Resources Research Institute in DS 2002. About 87 wild rice accessions were treated in PEG 8000 solution and planted in pots experiment with wax-petrolatum layer as base of pots to identified osmotic potential and root penetration ability of seedlings. The experiment was arranged randomized block design with three replications. The result of the experiment showed that only two accession of Oryza glaberrima and O. nivara were sprouted in 32.5% conc. of PEG 8000 solution. Root penetration ability of the wild rice were recorded i.e. O. glumaepatula, O. glaberrima, O. nivara, O. punctata, and O. rufipogon. O. glaberrima 101297 is expected to be the one of the best wild rice accession for drought tolerant, beside its shortest maturity date and having good characters