Admasu Engda, Engdawork

Brunelli, Claudio

Lynen, Frederic

Sandra, Patrick

English

Ghent University Academic Bibliography

  1  Effects of sample solvents on peak shape on early eluting compounds in HPLC, and EFLC/SFC  separations  Engdawork Admasu1, Frederic Lynen1, Claudio Brunelli3 and Pat Sandra1, 2, *  1Pfizer Analytical Research Centre, Ghent University, Krijgslaan 281, B-9000 Ghent, Belgium 2Research Institute for Chromatography, Kennedypark 26, B-8500 Kortrijk, Belgium 3Pfizer Global R&D, Analyst R&D, Sandwich CT13 9NJ, Kent, England  Presentation : Poster  Name of presenter: Engdawork Admasu (engdawork.engda@Ugent.be, aengd@yahoo.com) *Corresponding author: Prof. Pat Sandra (pat.sandra@richrom.com)   Abstract  As preparative chromatography was the first "killer application" for supercritical fluid chromatography (SFC) and the use of CO2 as a mobile phase and mobile phase component. Developing a scalable, fast way for  separation methods with enhanced fluid chromatography (EFLC) and SFC is  an integral element of the progress. In studying the effect of sample solvents  for EFLC/SFC, we selected polar modifier solvents such as water and methanol. Such polar solvents will produce possible splitting of early eluting peaks while working with EFLC /SFC in using similar injection volumes  and mobile phase composition compared to HPLC. The separation of standard test mixtures of polyaromatic compounds were used to evaluate the effect on early eluting compounds. Naphthalene peak showed distortion and further splitting as the mobile phase composition gets a  higher percent of CO2. For better chromatographic advantages while working in EFLC and SFC, a sample should be dissolved in the less polar solvent as much as possible consistent with the mobile phase composition. The use of pre-heated mobile phase is also another suggestion for handling polar mobile phase solvents at elevated temperature to minimize problems with early eluting peaks shape.  Keywords: EFLC, SFC, water, methanol, sample solvents  

Effects of sample solvents on peak shape on early eluting compounds in HPLC, and EFLC/SFC separations

https://biblio.ugent.be/publication/4100434/file/4100435.pdf

Effects of sample solvents on peak shape on early eluting compounds in HPLC, and EFLC/SFC separations

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Ghent University Academic Bibliography