Alonso, D.

Barchi, L.

Gaccione, L.

Giuliano, G.

Lanteri, S.

Portis, E.

Prohens, J.

Rotino, G. L.

Sulli, M.

Toppino, L.

English

Institutional Research Information System University of Turin

Proceedings of the LXV SIGA Annual CongressPiacenza, 6/9 September, 2022ISBN: 978-88-944843-3-5Poster Communication Abstract – 5.28 METABOLIC GWAS OF EGGPLANT PEEL COMPOSITION IN A WORLDWIDECOLLECTIONSULLI M.*, BARCHI L.**, GACCIONE L.**, TOPPINO L.***, ALONSO D.****,LANTERI S.**, PORTIS E.**, ROTINO G. L.***, PROHENS J.****, GIULIANO G.* *) ENEA, Casaccia Res Ctr, Roma 00123, Italy**) DISAFA, Plant Genetics and Breeding - University of Turin, 10095Grugliasco, Italy***) CREA, Research Centre for Genomics and Bioinformatics, 26836 MontanasoLombardo (LO), Italy****) Universitat Politècnica de València, 46022 Valencia, SpainSolanum melongena L., metabolic profiling, GWASWith a global production of 57 Mt in 2020 (FAOSTAT), eggplant (Solanum melongena L.) represents one of the most important vegetable crops.Eggplant fruits accumulate vitamins, phenolics and antioxidants, providingsignificant nutritional benefits, whose health protective effects andnutraceutical uses are recently becoming recognized. In the frame of theG2P-SOL project, we developed a collection of 322 homogeneous, non-segregant S. melongena accessions, as well as representatives of wildspecies belonging to the primary, secondary and tertiary genepools,representing the genetic diversity of over >3,600 accessions from worldwidegenebanks. The collection was subjected to resequencing at 20X, resultingin >51M high quality SNPs. Commercially mature fruits were harvested fromtwo independent field trials (Montanaso Lombardo, Italy and Valencia,Spain), sampled separately for peel and flesh, and semi-polar metaboliteswere analyzed by High Resolution LC-MS. Around 80 metabolites wereannotated for the peel on the basis of accurate masses, MS2 patterns,comparison with authentic standards and/or literature data. Peel extractsof the entire collection were analyzed, and quantitative data weregenerated. Association analysis between > 1.2M SNPs and the BLUP value ofeach accession for each metabolite was performed using the GAPIT R package,using MLMM, FarmCPU and BLINK models to compare their strength and power ofassociation detection. The multi-environment analysis resulted in 260mQTLs. Fifty-eight metabolites, including compounds with described healthbenefits (polyphenolic acids, anthocyanins, flavonols) or promotingbiotic/abiotic plant stress responses (polyamine conjugates,glycoalkaloids, citric acid), showed a heritability>0.6. The genetic basisof the mQTLs determining fruit quality are being investigated.

Metabolic GWAS of eggplant peel composition in a  worldwide collection

https://iris.unito.it/bitstream/2318/1879045/1/Metabolic_GWAS_of_eggplant_peel_composition_in_a_worldwide_collection.pdf

Metabolic GWAS of eggplant peel composition in a worldwide collection

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