Investigations into the use of long-wavelength fluorigenic substrates, which are
non- or weakly fluorescent but are converted by an appropriate enzyme to a
highly fluorescent product, have been carried out in order to determine several
different enzymes and their inhibitors.
Certain nonfluorescent compounds based on long-wavelength xanthenes and
oxazines dyes were synthesised. Amongst these, naphthofluorescein diphosphate
and naphthofluorescein mono-phosphate has been demonstrated
to be new fluorigenic substrates for alkaline phosphatase. The hydrolysis
product of these substrates can be used for trace analyses using diode laser
based fluorescence detection. [Continues.
