The relationship between particle mass and the number of ambient air particles for the submicrometer size range was examined using a Tapered Element Oscillating Microbalance to determine the mass concentration, and a Scanning Mobility Particle Sizer to determine the volume concentration and total number of particles. After validating the techniques through their application to the estimation of submicrometer particle density for two laboratory generated aerosols of known bulk density (Sodium Chloride and Di-2-ethylhexyl-sebacate), the submicrometer fraction of laboratory generated Environmental Tobacco Smoke and ambient air were examined and an estimate of the average submicrometer particle densities for these aerosols found to be 1.18 g cm-3 and 1.7 g cm-3 respectively