Associations of transcription factor 7-Like 2 (TCF7L2) gene
polymorphism in patients of type 2 diabetes mellitus from Khyber
Pakhtunkhwa population of Pakistan
Background: Type 2 diabetes mellitus (T2DM) is the most prevalent
component of metabolic syndrome. Environmental factors and various
complex genes like transcription factor 7-like 2 (TCF7L2) gene have
involved in the disease development. Objective: To determine TCF7L2
genetic association (rs7903146C/T and rs12255372G/T) in T2DM patients
of Khyber Pakhtunkhwa population of Pakistan. Subjects and methods:
This study comprised of 176 subjects including 118 T2DM patients and 58
healthy controls. Genomic DNA was extracted and genotype of common
variants (rs7903146 C/T and rs12255372 G/T) was carried out by
amplification-refractory mutation system (ARMS)-PCR of sequence
specific oligonucleotides. Results: The distribution of genotype of
TCF7L2 SNPs (rs7903146 C/T and rs12255372 G/T) was significantly
associated with T2DM as compared to the controls (p <0.0001). The
genetic models of the rs7903146 (C/T) and rs12255372 (G/T) SNPs were
significantly associated between cases and controls (p <0.0001). On
the other hand, the significant association was observed between the
two SNPs and different biochemical parameters like serum fasting
glucose, lipid profile, creatinine and blood HbA1c levels (p <0.05).
Conclusion: It is concluded that the SNPs of the TCF7L2 gene are
significantly associated with T2DM disease susceptibility in the
population of Khyber Pakhtunkhwa of Pakistan