Tibouchina sellowiana (Cham.) Cogn,: rooting, anatomy and biochemical analyses in the four seasons

Abstract

O presente trabalho buscou verificar os efeitos da aplica\ue7\ue3o de diferentes concentra\ue7\uf5es do \ue1cido indolbut\uedrico (AIB), em talco e em solu\ue7\ue3o, no enraizamento de estacas caulinares de Tibouchina sellowiana (Cham.) Cogn. coletadas nas quatro esta\ue7\uf5es do ano, bem como suas varia\ue7\uf5es anat\uf4micas e bioqu\uedmicas. Estacas semilenhosas foram tratadas com 0, 1500 e 3000 mg L-1 de \ue1cido indolbut\uedrico (AIB), em talco e solu\ue7\ue3o 50% alco\uf3lica. Ap\uf3s 65 dias em casa-de-vegeta\ue7\ue3o, foram avaliados a porcentagem de estacas enraizadas, o n\ufamero e o comprimento das ra\uedzes, a porcentagem de estacas com calos e a sobreviv\ueancia. O uso do AIB mostrou-se eficiente na indu\ue7\ue3o radicial, sendo indiferente a forma em que foi aplicado. As esta\ue7\uf5es mais promissoras foram a primavera (78,75%) com 3.000 mg L-1 ou mg kg-1 de AIB e o ver\ue3o (63,12%) com 1.500 mg L-1 ou mg kg-1 de AIB. Das sec\ue7\uf5es feitas na base das estacas, no momento da estaquia, n\ue3o foi detectada nenhuma poss\uedvel barreira anat\uf4mica ou diferen\ue7a estrutural que promovesse varia\ue7\uf5es no enraizamento. An\ue1lises bioqu\uedmicas feitas das estacas revelaram que as maiores concentra\ue7\uf5es de a\ue7\ufacares totais foram obtidas no inverno (83,21 mg g-1 de tecido) e no outono (72,79 mg g-1), as esta\ue7\uf5es que tamb\ue9m apresentaram as maiores concentra\ue7\uf5es de prote\uednas (4,69 e 3,95 mg g-1 respectivamente).The present work tried to verify the effects of application of different concentration of indolebutyric acid (IBA) in talc in solution, in the rooting of cutting of Tibouchina sellowiana (Cham.) Cogn. collected in the four seasons, as well as its anatomical and biochemical variation. The semiharwood cuttings were treated with 0, 1500 and 3000 mg L-1 indolebutyric acid (IBA), in powder and alchoolic solutions. After 65 days in a greenhouse, rooting percentage, length and number of roots, cutting with callus percentage and survival, were evaluated. The use of the IBA was efficient in the induction radicial, being indifferent the form in which it was applied. The most promising stations in the formation of the system radicial were the spring (78,75%) with 3000 mg L-1 or mg kg-1 IBA and the summer (63,12 %) with 1500 mg L-1 or mg kg-1 IBA. (82,50%) with 3000 mg kg-1 IBA. Considering the cutting in the base of the stakes, in the moment of the cutting, no possible anatomical barrier or structural difference were detected so that it should promoted variations in the answers of the rooting uring the four seasons. Biochemical analyses done from the cuttings collected in the four seasons revealed that the largest concentrations of total sugars were obtained in the winter (83,21 mg g-1) and in the autumn (72,79 mg g-1). The same stations also presented the largest protein concentrations (4,69 and 3,95 mg g-1 respectively)