CARVALHEIRA, J.

COSTA, C. N.

LOPES, P. S.

SILVA, A. A.

SILVA, D. A.

SILVA, H. T.

THOMPSON, G.

VERONEZE, R.

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136 EAAP – 73rd Annual Meeting, Porto, Portugal, 2022SNP substitution effects for SCS changes across environmental gradients in Portuguese dairy cattleA.A. Silva1, D.A. Silva1, P.S. Lopes2, H.T. Silva2, R. Veroneze2, G. Thompson3,4, C.N. Costa5 and J. Carvalheira3,41FCAV-UNESP, Animal Science, R. Prof. P.D. Castellane, 14884-900 Jaboticabal, Brazil, 2U.F.Viçosa, Animal Science,UFV, 36570-000 Viçosa, Brazil, 3ICBAS-U.Porto, R. J.V. Ferreira, 228, 4050-313 Porto, Portugal, 4BIOPOLIS-CIBIO-U.Porto, R. Padre A. Quintas, 4485-661, Vairão, Portugal, 5Embrapa Gado de Leite, Embrapa, 36038-330 Juiz de Fora,Brazil; jgc3@cibio.up.ptSeverl GWAS reports for milk relted trits hve been performed, however, the use of this pproch in  context ofG by E, is yet scrce. We imed t identifying puttive cndidte genes ssocited with milk yield (MY) nd somticcell score (SCS) according to an environmental gradient (EG). A total o 4.6 million test-day records oMYand SCSrom Portuguese Holstein cows were analysed. The data included 1,537 genotyped animals or 38,615 SNPs. First,the herd-test-day (HTD) eects were estimated using an autoregressive test-day model. Then, the solutions o HTDeect were used as an EG in a reaction norm model under ssGLBUP approach. The SNP eects or MY and SCSwere clculted by bck solving the genomic breeding vlues for ech EG nd the proportion of genetic vrinceexplined by 100 kb SNP windows ws lso computed. For MY, the SNP effects were lmost constnt cross EG,indicating the absence o SNP by environment interaction. Nevertheless, or SCS, higher changes in the magnitudeo SNP eects were observed among EG. Based on signicant SNP windows or MY and SCS between the extremeEG, candidate genes were mapped. For MY (SCS), we identied 25 (33) candidate genes unique or less avourableand 11 (10) candidate genes or more avourable EG. For SCS, candidate genes SOX14, HGF, and GPRIN1 wereidentied only in less avourable EG while NAALADL2, ELMO2, and ZNF830 were identied exclusively in moreavourable EG. NAALADL2 gene or example, is related to response to bacterium and HGF is related to regulationof MAPK cscde, importnt in stress responses under pthologicl conditions. Meningful differences in the geneticarchitecture o SCS across EG were revealed in this study and may contribute to a better understanding o the SCSgenetic control, permitting the design o better strategies or genomic selection in specic dairy herd environmentsin Portugl.Comparison of longissimus lumborum muscle transcriptomes in Romanov and Polish Merino sheepE. Grochowska1, Z. Cai2 and M. Grguła-Kania31Bydgoszcz University of Science and Technology, Department of Animal Biotechnology and Genetics, Mazowiecka 28,85-084, Poland, 2Aarhus University, Center or Quantitative Genetics and Genomics, Blichers Allé 20, Postboks 50,8830 Tjele, Denmark, 3University of Life Sciences in Lublin, Institute of Animal Breeding and Biodiversity Conservation,Akademicka 13, 20-950 Lublin, Poland; grochowska@pbs.edu.plMeat quality is a complex trait, which is dicult to improve by applying only traditional breeding methods. Analysisomuscle tissue transcriptomes can enhance our understanding o the genes controlling meat quality in dierent sheepbreeds. The study imed to investigte the longissimus lumborum (LL) muscle trnscriptomes in Romnov (ROM)nd Polish Merino (PM) sheep to revel differences in the expression of genes, including those ssocited with metquality. The experiment was conducted on 3 ROM and 3 PM emale lambs. Samples o LL muscle were collectedimmediately ater commercial slaughter. Dierential expression analysis was conducted using DESeq2 v.1.34.0package. ClusterProler v4.2.2 sotware was applied or enrichment analysis o dierentially expressed genes (DEG).A total o 381 mRNA transcripts, including 237 up- and 144 downregulated transcripts, were dierentially expressedin ROM relative to PM lambs. The signicant GO terms (Padj<0.05) or all DEGs included 9 GO terms in the categoryo a cellular component. Regarding the up-regulated genes dataset, a total o 7 GO terms were signicantly enriched(Pdj<0.05), including 5 nd 2 GO terms in the ctegories of cellulr component, nd moleculr function, respectively.The urther enrichment analysis among the 381 DEGs revealed 23 signicantly enriched (Padj<0.05) KEGG pathways.These results provide  foundtion for identifying cndidte genes nd further develop the theoreticl bsis for newbreeding strategies to optimize the production perormance and meat quality o the two sheep breeds. Our outcomesprovide lso the foundtion for further use of these breeds for crossbreeding. This work ws supported by theMinistryo Science and Higher Education rom the ‘Innovation Incubator 2.0’ programme (grant no. 8/1/2019/UTP) and bythe Polish Ntionl Agency for Acdemic Exchnge (grnt no. PPI/APM/2019/1/00003).Session 03 Poster 15Session 03 Poster 14

SNP substitution effects for SCS changes across environmental gradients in Portuguese dairy cattle.

http://www.alice.cnptia.embrapa.br/bitstream/doc/1146497/1/SNP-substitution-effects-for-SCS-changes-across-environmental-gradients-in-Portuguese-dairy-cattle.pdf

SNP substitution effects for SCS changes across environmental gradients in Portuguese dairy cattle.

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